pg. 20 Rev E 1/14/15
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
If your samples contain soybean products or >5% fat, before doing the NDF analysis in
the ANKOM
2000
, you will need to do a pre-extraction. For samples containing non-roasted
soybean or >5% fat, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for twelve
hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
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